🧪 Validation Methods

🔬 Laboratory Validation

Molecular Binding Studies

• Direct Measurement: AlF/AlF3 protein binding
• Kinetic Analysis: Association/dissociation rates
• Structural Analysis: X-ray crystallography, cryo-EM
• Quantification: Binding constants and Hill coefficients

Cellular Response Studies

• Cell Cultures: Neural cells with varying concentrations
• Misfolding Rates: Protein aggregation measurement
• Damage Assessment: Cellular dysfunction quantification
• Viability Testing: Cell survival analysis

🧠 Tissue Analysis

Penetration Studies

• Ex Vivo Testing: Brain tissue penetration analysis
• Concentration Mapping: Spatial distribution tracking
• Correlation Analysis: Concentration vs. aggregation
• BBB Transit: Transport mechanism verification

📈 Clinical Correlations

Epidemiological Evidence

• Geographic Correlation: Exposure vs. prevalence
• Dose-Response: Concentration relationship
• Temporal Patterns: Exposure timing effects
• Population Studies: Risk factor analysis

Therapeutic Response

• Level Monitoring: Real-time compound tracking
• Clearance Correlation: Treatment response patterns
• Biomarker Changes: Protein folding improvements
• Outcome Measurement: Cognitive function tests